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multiple human adult brain tissue northern blots  (TaKaRa)


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    TaKaRa multiple human adult brain tissue northern blots
    Multiple Human Adult Brain Tissue Northern Blots, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+adult+multiple+tissue+northern+blots/Human+Brain+Total+RNA/us11932908-1087-19-31
    Average 94 stars, based on 1153 article reviews
    multiple human adult brain tissue northern blots - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes.
    Article Snippet: .. Northern Analyses—The expression patterns for mouse and human UDP-glucose dehydrogenase were determined through hybridization of cDNA probes for the respective genes to mouse embryo and human adult multiple tissue Northern blots (CLONTECH, Palo Alto, CA). ..

    Article Title: DLP, a Novel Dim1 Family Protein Implicated in Pre-mRNA Splicing and Cell Cycle Progression
    Article Snippet: RT-PCR and Northern Blotting—Total cellular RNA was isolated using the TRIzol reagent (Invitrogen), and first strand cDNA was prepared using the ProtoScriptTM first strand cDNA synthesis kit with a dT23 VN Primer (New England Biolabs, Beverly, MA). cDNA synthesis was checked by PCR with human glyceraldehyde-3-phosphate dehydrogenase primers as a positive control. .. Human adult multiple tissue Northern blots (Clontech) were used to detect human DLP mRNA expression profile. .. A 447-bp fragment spanning 287–736 bp of human DLP cDNA was prepared as a probe and labeled by random priming with [ -32P]dCTP (Amersham Biosciences).

    Article Title: Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells.
    Article Snippet: Vertebrates have muscle and non-muscle isozymes of adenylosuccinate synthetase (AdSS, EC 6.3.4.4), which catalyzes the first committed step in AMP synthesis.. A novel muscle isozyme of adenylosuccinate synthetase, human AdSSL1, is identified from human bone marrow stromal cells.. AdSSL1 is 98% identical to mouse muscle type AdSS1 and contains conserved sequence and structural features of adenylosuccinate synthetase.

    Article Title: Cloning and Characterization of Siglec-10, a Novel Sialic Acid Binding Member of the Ig Superfamily, from Human Dendritic Cells
    Article Snippet: RT-PCRs with the primers 59-TGGCTCAGAAGCGGAATC-39 (forward) and 59-CCTCATTGGAACTTGACTTCTGC-39 (backward) specific for Siglec-10 cDNA were subjected to denaturing (95 °C for 15 s), annealing (57 °C for 30 s), and extension (72 °C for 30 s) for 28 cycles using a Perkin-Elmer GeneAmp PCR System 9600. .. Human adult multiple tissue Northern blots (CLONTECH) were used to detect mRNA expression by Northern blot analysis. .. The RTPCR products mentioned above were cloned into a T/A vector for sequencing and labeled by random priming with [a-32P]dCTP (50 mCi, 3000 Ci/mmol, Amersham Pharmacia Biotech) as a probe.

    Article Title: RNA Processing and Modification Protein, Carbon Catabolite Repression 4 (Ccr4), Arrests the Cell Cycle through p21-dependent and p53-independent Pathway
    Article Snippet: .. Human adult multiple tissue Northern blots (Clontech) or cancer profiling array (Clontech) were used, and a 500-bp probe of human ccr4d cDNA was labeled by random priming with [α- 32 P]dCTP (Amersham Biosciences). .. Hybridization was performed in ExpressHyb hybridization solution (Clontech) according to the manufacturer's instructions.

    Expressing:

    Article Title: Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes.
    Article Snippet: .. Northern Analyses—The expression patterns for mouse and human UDP-glucose dehydrogenase were determined through hybridization of cDNA probes for the respective genes to mouse embryo and human adult multiple tissue Northern blots (CLONTECH, Palo Alto, CA). ..

    Article Title: DLP, a Novel Dim1 Family Protein Implicated in Pre-mRNA Splicing and Cell Cycle Progression
    Article Snippet: RT-PCR and Northern Blotting—Total cellular RNA was isolated using the TRIzol reagent (Invitrogen), and first strand cDNA was prepared using the ProtoScriptTM first strand cDNA synthesis kit with a dT23 VN Primer (New England Biolabs, Beverly, MA). cDNA synthesis was checked by PCR with human glyceraldehyde-3-phosphate dehydrogenase primers as a positive control. .. Human adult multiple tissue Northern blots (Clontech) were used to detect human DLP mRNA expression profile. .. A 447-bp fragment spanning 287–736 bp of human DLP cDNA was prepared as a probe and labeled by random priming with [ -32P]dCTP (Amersham Biosciences).

    Article Title: Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells.
    Article Snippet: Vertebrates have muscle and non-muscle isozymes of adenylosuccinate synthetase (AdSS, EC 6.3.4.4), which catalyzes the first committed step in AMP synthesis.. A novel muscle isozyme of adenylosuccinate synthetase, human AdSSL1, is identified from human bone marrow stromal cells.. AdSSL1 is 98% identical to mouse muscle type AdSS1 and contains conserved sequence and structural features of adenylosuccinate synthetase.

    Article Title: Cloning and Characterization of Siglec-10, a Novel Sialic Acid Binding Member of the Ig Superfamily, from Human Dendritic Cells
    Article Snippet: RT-PCRs with the primers 59-TGGCTCAGAAGCGGAATC-39 (forward) and 59-CCTCATTGGAACTTGACTTCTGC-39 (backward) specific for Siglec-10 cDNA were subjected to denaturing (95 °C for 15 s), annealing (57 °C for 30 s), and extension (72 °C for 30 s) for 28 cycles using a Perkin-Elmer GeneAmp PCR System 9600. .. Human adult multiple tissue Northern blots (CLONTECH) were used to detect mRNA expression by Northern blot analysis. .. The RTPCR products mentioned above were cloned into a T/A vector for sequencing and labeled by random priming with [a-32P]dCTP (50 mCi, 3000 Ci/mmol, Amersham Pharmacia Biotech) as a probe.

    Hybridization:

    Article Title: Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes.
    Article Snippet: .. Northern Analyses—The expression patterns for mouse and human UDP-glucose dehydrogenase were determined through hybridization of cDNA probes for the respective genes to mouse embryo and human adult multiple tissue Northern blots (CLONTECH, Palo Alto, CA). ..

    Labeling:

    Article Title: RNA Processing and Modification Protein, Carbon Catabolite Repression 4 (Ccr4), Arrests the Cell Cycle through p21-dependent and p53-independent Pathway
    Article Snippet: .. Human adult multiple tissue Northern blots (Clontech) or cancer profiling array (Clontech) were used, and a 500-bp probe of human ccr4d cDNA was labeled by random priming with [α- 32 P]dCTP (Amersham Biosciences). .. Hybridization was performed in ExpressHyb hybridization solution (Clontech) according to the manufacturer's instructions.



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    Fig. 2 Expression of nSPN mRNA transcript in <t>human</t> <t>tissues.</t> <t>Northern</t> <t>blots</t> containing 2 mg of poly-A+ RNA from the indicated human tissues were hybridized with nSPN-specific oligonucleotide. An arrow indicates the 1.5-kb nSPN mRNA transcript. Molecular size markers are indicated on the left (kb)
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    Fig. 2 Expression of nSPN mRNA transcript in human tissues. Northern blots containing 2 mg of poly-A+ RNA from the indicated human tissues were hybridized with nSPN-specific oligonucleotide. An arrow indicates the 1.5-kb nSPN mRNA transcript. Molecular size markers are indicated on the left (kb)

    Journal: Skeletal muscle

    Article Title: Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F.

    doi: 10.1186/s13395-017-0127-9

    Figure Lengend Snippet: Fig. 2 Expression of nSPN mRNA transcript in human tissues. Northern blots containing 2 mg of poly-A+ RNA from the indicated human tissues were hybridized with nSPN-specific oligonucleotide. An arrow indicates the 1.5-kb nSPN mRNA transcript. Molecular size markers are indicated on the left (kb)

    Article Snippet: Adult human multiple tissue Northern blots (Clontech Inc.) containing 2 μg of poly-A+ RNA per lane were probed with oligonucleotides spanning exon 1 and exon 4 as described previously [1] and in Table 1.

    Techniques: Expressing, Northern Blot

    Northern blots of poly(A)+ RNA from different human tissues were probed with a 491-bp fragment of human SERT cDNA (A). Arrows indicate RNA size markers (in bp). Higher exposure of ileum lane is shown in B.

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: Function, expression, and characterization of the serotonin transporter in the native human intestine

    doi: 10.1152/ajpgi.00354.2007

    Figure Lengend Snippet: Northern blots of poly(A)+ RNA from different human tissues were probed with a 491-bp fragment of human SERT cDNA (A). Arrows indicate RNA size markers (in bp). Higher exposure of ileum lane is shown in B.

    Article Snippet: To ascertain the presence of mRNA species encoding SERT in various human intestinal tissues, commercially available human multiple-tissue Northern blots (BioChain) were probed with 32 P-labeled 491-bp human SERT cDNA fragment.

    Techniques: Northern Blot